{"id":4048,"date":"2021-07-20T09:16:01","date_gmt":"2021-07-20T09:16:01","guid":{"rendered":"https:\/\/www.molecular-view.com\/?p=4048"},"modified":"2021-09-16T15:46:24","modified_gmt":"2021-09-16T15:46:24","slug":"thyroid-fna-diagnostics-study","status":"publish","type":"post","link":"https:\/\/www.molecular-view.com\/el\/thyroid-fna-diagnostics-study\/","title":{"rendered":"Thyroid FNA diagnostics study"},"content":{"rendered":"

Thyroid FNA diagnostics study<\/h3>\t\t\t

\u201cA 9-gene molecular signature enhances diagnosis of thyroid nodular goiter\u201d<\/strong><\/p>\n

Tsoumalis G., Kalimani E., Sarantopoulou C., Moraitis D., and Nikolopoulos SN.<\/strong><\/p>\n

Center for Molecular Analysis and Research SA, Agrinio, Greece<\/em><\/p>\n

 <\/p>\n

\u0399\u03c3\u03c4\u03bf\u03c1\u03b9\u03ba\u03cc<\/strong>
\nAlthough FNA is currently considered to be the best initial diagnostic test for evaluation of a thyroid
\nnodule, it cannot always discriminate between benign and malignant thyroid tumors. To address this
\nissue, we developed a molecular assay for thyroid FNAs that tests both the expression of genes that
\nbecoming expressed in cancerous thyroid tissues, as well as the presence of the oncogenic BRAF V600E
\nmutation.<\/p>\n

Methodology<\/strong>
\nWe used a cohort of 102 cases (105 nodules), 88 women and 14 men, aged 46,6 +\/- 15 years old,
\npresented with nodular or multinodular, non toxic goiter. Thyroid ultrasound guided FNABs were
\nperformed. Tissue from 2 aspirations of the needle were used for a cytological examination. A third
\naspiration was transferred into a tube containing nucleic acids stabilization buffer. A molecular 9-gene
\nsignature that tests the expression of 8 genes encoding cell surface receptors and oncogenes and the
\npresence of BRAF V600E mutation was performed, by quantitative real-time PCR.<\/p>\n

Results<\/strong>
\nA positive 9-gene signature was obtained in 20 cases (19%). Agreement between gene signature and
\ncytology assessment was found in 102 out of 105 nodules (97%). Three cases (15.7% of all positive
\ncases) were positive for malignancy by gene signature and negative by cytological criteria. However, all 3
\ncases were found to be positive for malignancy after surgery, which underscores the value of this
\nmolecular assessment. We have validated this gene signature with surgical samples from 29
\nthyroidectomies. BRAF V600E mutation was absent only in one case of a positive biopsy.<\/p>\n

Conclusion<\/strong>
\nWe developed a combined gene expression and mutation analysis assay as an accurate tool for
\ndetermining the presence\/absence of malignancy in thyroid FNAs. This methodology will enhance the
\ndiagnosis of thyroid nodules, particularly in those cases in which it is necessary to resolve uncertainties.<\/p>\n

 <\/p>\n

Oral presentation,<\/p>\n

Presented at\u00a0American Thyroid Association<\/strong>\u00a0Conference, Quebec, Canada, September 21 2012<\/p>\n

 <\/p>\n

<\/p>\n<\/div>\n\t\t<\/div><\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

\u201cA 9-gene molecular signature enhances diagnosis of thyroid nodular goiter\u201d Tsoumalis G., Kalimani E., Sarantopoulou C., Moraitis D., and Nikolopoulos SN. Center for Molecular Analysis and Research SA, Agrinio, Greece   Background Although FNA is currently considered to be the best initial diagnostic test for evaluation of a thyroid nodule, it cannot always discriminate between<\/p>","protected":false},"author":1,"featured_media":4080,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[29],"tags":[],"class_list":["post-4048","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-29"],"_links":{"self":[{"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/posts\/4048","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/comments?post=4048"}],"version-history":[{"count":2,"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/posts\/4048\/revisions"}],"predecessor-version":[{"id":4071,"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/posts\/4048\/revisions\/4071"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/media\/4080"}],"wp:attachment":[{"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/media?parent=4048"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/categories?post=4048"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.molecular-view.com\/el\/wp-json\/wp\/v2\/tags?post=4048"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}